FLIM System for Laser Scanning Microscopes

Upgrade your laser scanning microscope to carry out fluorescence lifetime imaging (FLIM) with these highly modular systems from Becker and Hickl. Available for multiphoton non-descanned detection (NDD), FLIM confocal, upright and inverted microscopes.

Becker and Hickl (B&H) have been supplying FLIM systems for laser scanning microscopes since 1998 and now offer highly modular FLIM systems that can be configured to upgrade any confocal laser scanning microscopes to carry out FLIM.

Based on B&H’s proprietary multidimensional time correlated single photon counting (TCSPC) technique, the FLIM systems are based on a picosecond blue or ultraviolet fiber coupled diode laser, a detector module (single, dual or multiwavelength) and electronics based on single TCSPC module with routing, or on a multimodule TCSPC system.

There are FLIM systems for one photon and multiphoton microscopes, and for confocal and non-descanned detection (NDD). Pulsed laser light is fed into the microscope with a single mode optical fiber. A high speed GaAsP detector, connected to the optical output of the scan head collects the output signal from the sample and, with TCSPC, allows a picture of the fluorescence lifetime of the sample to be built up.

Becker and Hickl supply upgrade kits for many popular microscope systems including:

Zeiss 710, 780 and 880 laser scanning microscopes, Leica SP2 and SP5 multiphoton microscopes, Zeiss 510 one photon and 510 nonlinear optical laser scanning microscopes, Olympus FV1000 confocal microscopes and Nikon C1, A1, TiE microscopes.


  • Multidimensional TCSPC technique
  • Excellent time resolution and sensitivity
  • Variety of detector modules available
  • Peripheral component interconnect (PCI) cards or laptop based systems available
  • Multiphoton NDD FLIM
  • Confocal FLIM
  • Single detector systems
  • Dual detector systems
  • Multispectral FLIM systems



This product could be used for the following applications:

  • Fluorescence lifetime imaging (FLIM)
  • Biological fluorescence
  • Two photon microscopy
  • Confocal microscopy


This product is manufactured by Becker and Hickl GmbH.

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