FLIM System for Leica SP2 and SP5 Microscopes

FLIM Systems for Leica Microscopes

Becker and Hickl's range of fluorescence lifetime imaging (FLIM) systems is expanded with the introduction of a non-descanned detection (NDD) unit for use with Leica SP2 and SP5 multiphoton microscopes.

Compared with detection through the confocal beam path, non-descanned detection yields a considerably higher efficiency for deep sample layers.

The non-descanned FLIM detectors are attached to the reflected light (RLD) port of the microscope. The FLIM electronics are contained in an extension box of a laptop computer. The FLIM data acquisition is controlled by the scan clock signals of the microscope. No changes in the microscope hardware or software are required for the NDD FLIM systems, which makes installation on any Leica SP2 or SP5 multiphoton microscope very easy.


  • Complete NDD FLIM
  • Time correlated single photon counting (TCSPC) data acquisition
  • Choice of FLIM detectors
  • High speed FLIM
  • High resolution FLIM
  • Multispectral FLIM



This product could be used for the following applications:

  • Fluorescence lifetime imaging (FLIM)
  • Two photon microscopy
  • Biological fluorescence


This product is manufactured by Becker and Hickl GmbH.

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