FEMTOSmart-Dual Two-Photon Microscope

The FEMTOSmart Dual microscope contains both galvanometric and resonant scanners, providing all advantages of the two imaging techniques. Using the two scanner functions in tandem is a perfect solution for photostimulation. The galvanometric scanner directs the laser to cells or subcellular components selectively. With the resonant scanner, you can follow the changes in the cells of a neural network, collecting imaging data simultaneously by high-speed frame scanning of the surrounding area. 

The FEMTOSmart series consists of fully customizable two-photon microscopes, which always fit perfectly to the customers’ needs. The special feature of these scopes is the elevated body, which can move in X, Y, and Z directions, providing ample room under the objective for the optimal positioning of your samples.

With the Galvo scanner you can zoom to tiny structures such as dendritic spines and jump quickly between these zoomed regions. In contrast, with the Resonant scanner you can capture images with high frame rate to follow rapid changes on the field of view (FOV).

Choose the best scanning mode which fits your imaging requirements. The Resonant scanner ensures high speed image acquisition up to 31 frames per second (fps) over the whole FOV whereas the Galvo scanner provides flexible region of interest (ROI) scanning opportunities.

Features

• In vivo deep brain imaging down to 850μm
• Galvo
  • 800x800μm field of view (FOV) (with a 20x objective lens)
  • Pixel dwell time adjustable 0.5μs - 10ms, pixel based averaging
• Resonant
  • 600x600μm (FOV) (with a 20x objective lens)
  • Dynamic pixel dwell time to avoid image distortion
• Minimised optical path length by patented travelling detector system
• Non-descanned, ultrasensitive GaAsP photomultiplier tubes (PMT) (>40% quantum efficiency)
• High signal-to-noise ratio
• Simultaneous detection of multiple wavelengths
• Custom designed optical elements for maximal transmission efficiency
• Control software with analysis and upgrade possibilities
• Parallel recording and analysis of electrophysiological data
• Complementary metal oxide semiconductor (CMOS) camera
• Compatibility with extended infrared wavelength range

Galvo and Resonant Advantages

The galvanometric scanner directs the laser to cells or subcellular components selectively. Selectivity is ensured by scanning along arbitrary line patterns, placed on cell bodies or dendritic segments. With the resonant scanner, you can follow the changes in the cells of a neural network, collecting imaging data simultaneously by high-speed frame scanning of the surrounding area. The timing of photostimulation and detector gating is controlled by protocols designed on the user-friendly GUI. The Tilting objective, as an optional module, can increase accessibility of the objective to the sample.

Datasheet

• FEMTOSmart Series Overview
• Femtonics Microscope Range
• Femtonics 3p Applications

Applications

This product could be used for the following applications:

• Deep brain imaging
• Calcium imaging
• Parallel electrophysiology

Manufacturer

This product is manufactured by Femtonics - Advanced Microscopy.