FEMTOSmart-Galvo Two-Photon Microscope

The FEMTOSmart-Galvo from the FemtoSmart series is a galvanometric scanner based two-photon microscopes which allows functional imaging focusing on the region of interest (ROI) for high speed and SNR. The galvo scanner combined with Femtonics' intelligent control software enables the user to select numerous scanning patterns covering widely distributed ROIs including multiple frame scanning, multiple line scanning and random-access point scanning.

Scanning only the ROI and skipping the background establish fast imaging speed to follow rapid changes and high signal-to-noise ratio to reveal more signals.

The galvanometric scanner – commonly called "galvo" – consists of two galvanometer based motor driven mirrors allowing arbitrary positioning of the focal spot. High accuracy and this positioning freedom support flexible approaches for ROI creation.

Features

  • In vivo deep brain imaging down to 850μm
  • 800x800μm field of view (FOV) (with a 20x objective lens)
  • 2D scanning modes
  • Pixel dwell time adjustable 0.5μs - 10ms, pixel based averaging
  • Minimised optical path length by patented travelling detector system
  • Non-descanned, ultrasensitive GaAsP photomultiplier tubes (PMT) (>40% quantum efficiency)
  • High signal-to-noise ratio
  • Simultaneous detection of multiple wavelengths
  • Custom designed optical elements for maximal transmission efficiency
  • Control software with analysis and upgrade possibilities
  • Parallel recording and analysis of electrophysiological data
  • Complementary metal oxide semiconductor (CMOS) camera
  • Compatibility with extended infrared wavelength range

Focusing on regions of interest for high speed and SNR

Multiple frame scanning allows focusing on cell bodies, multiple line scanning enables us to follow action potentials along dendrites, random-access point scanning allows for the measurement or photostimulation of subcellular components with the highest temporal resolution. Our software features (real-time display, analysis functions, F/F calculations, the integrated parallel data acquisition of electrical recordings) aid the understanding of physiological processes in your research focus.

Scanning modes of FEMTOSmart Galvo

Multiple line scanning

Multiple line scanning has been developed for researchers aiming to resolve dendritic and even spine activity of neurons with a near real-time measurement mode. During this scanning mode, the X and Y mirrors direct the laser beam in a flexible way along straight lines or complex curves. The scanner spends most of its time collecting signals from these lines, skipping intermediate sections between them. Therefore, the scanning speed and the signal-to-noise ratio (SNR) of the signals from the multi-site ROIs increases 3- to 4-fold compared to frame scanning.

Folded frame scanning

This patented method enables users to image a confined area along a line, where the shape of the selected regions can be straight or curved. This advanced scanning method is useful for imaging single cell bodies in different regions of the specimen, or following events along winding dendrites with their protrusions, even in the tissues of moving animals.

Datasheet

Applications

This product could be used for the following applications:

  • Deep brain imaging
  • Calcium imaging
  • Parallel electrophysiology
  • Photostimulation
  • Intravital imaging

Manufacturer

This product is manufactured by Femtonics - Advanced Microscopy.

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