The FEMTOSmart-Galvo from the FemtoSmart series is a galvanometric scanner based two-photon microscopes which allows functional imaging focusing on the region of interest (ROI) for high speed and SNR. The galvo scanner combined with Femtonics' intelligent control software enables the user to select numerous scanning patterns covering widely distributed ROIs including multiple frame scanning, multiple line scanning and random-access point scanning.
Scanning only the ROI and skipping the background establish fast imaging speed to follow rapid changes and high signal-to-noise ratio to reveal more signals.
The galvanometric scanner – commonly called "galvo" – consists of two galvanometer based motor driven mirrors allowing arbitrary positioning of the focal spot. High accuracy and this positioning freedom support flexible approaches for ROI creation.
Multiple frame scanning allows focusing on cell bodies, multiple line scanning enables us to follow action potentials along dendrites, random-access point scanning allows for the measurement or photostimulation of subcellular components with the highest temporal resolution. Our software features (real-time display, analysis functions, F/F calculations, the integrated parallel data acquisition of electrical recordings) aid the understanding of physiological processes in your research focus.
Multiple line scanning has been developed for researchers aiming to resolve dendritic and even spine activity of neurons with a near real-time measurement mode. During this scanning mode, the X and Y mirrors direct the laser beam in a flexible way along straight lines or complex curves. The scanner spends most of its time collecting signals from these lines, skipping intermediate sections between them. Therefore, the scanning speed and the signal-to-noise ratio (SNR) of the signals from the multi-site ROIs increases 3- to 4-fold compared to frame scanning.
This patented method enables users to image a confined area along a line, where the shape of the selected regions can be straight or curved. This advanced scanning method is useful for imaging single cell bodies in different regions of the specimen, or following events along winding dendrites with their protrusions, even in the tissues of moving animals.
This product could be used for the following applications:
This product is manufactured by Femtonics - Advanced Microscopy.
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